Ras induces FucT-VII in Jurkat cells. Jurkat cells were infected with V-H-Ras, Raf-BXB, or control retroviruses and analyzed 3 days after infection. (A) HECA-452 staining is induced selectively on Ras/GFP-high cells; Ras/GFP⁺ cells remain HECA-452^–. (B) Inhibition of Ras-induced FucT-VII expression by MAPK inhibitors. Jurkat cells were infected with Ras retrovirus, and inhibitors were included at the time of infection. Inhibitors are exactly as in Figure 1. (C) Raf-BXB infection alone leads to minimal HECA-452 staining. (D) Effects of MAPK inhibitors on Raf-BXB–induced FucT-VII expression. Inhibitors are as in previous igures; MerckC was not included in this series of experiments. (E) Induction of FucT-VII by Raf-BXB in the presence of SB203580 is limited to the highest Raf-BXB–expressing cells. *P < .05 versus treatment with no inhibitors.