Reduced homing capacity of WASP-deficient bone marrow cells as compared with wild-type cells. Lethally irradiated female mice received transplants of a mixture of 10 × 106 wild-type and 10 × 106 WASP-deficient bone marrow cells. The bone marrow cells were stained with 2 different dyes: wild-type mice were stained with CSFE, and WASP-deficient cells with PKH26, or vice versa. (A) At 24 hours after the graft, bone marrow and spleen were harvested from each recipient, and nucleated cells were analyzed by flow cytometry to evaluate the proportion of red and green cells in the 2 hematopoietic tissues. Results shown are means ± SD of 4 experiments done with 3 mice in each group. (B) Bone marrow and spleen cells of recipient mice were used for progenitor assays, and 20 colonies per mouse were picked and monitored by Y and neo PCR to determine the origin of the cell that gives rise to the colony. Seven recipient mice were analyzed, and 40 colonies per mouse were harvested (20 from the bone marrow and 20 from the spleen; a total of 140 colonies from the bone marrow and 140 from the spleen were analyzed). Results shown are means ± SD of 7 analyzed mice.
