Figure 3.
Representative ethidium bromide–stained gel electrophoresis of LDI-PCR of the BCL6. (A) The sizes of the LDI-PCR products are unique to each case. (B) A gain or a loss of partner/BCL6 fusion was observed in the paired diagnosis-transformation samples. The LDI-PCR products represent fusions either on the der(3) or der(partner), and partner genes or loci identified by sequencing analysis of the products are indicated at the bottom. An aliquot of 2 to 10 μL was loaded in each lane and electrophoresed through a 0.7% agarose gel. HindIII-digested DNA was used as a molecular weight marker. D indicates at the diagnosis; T, at the transformation.