Figure 4.
![Figure 4. Microsatellite instability in CFUs isolated from mice receiving MSH2–/– bone marrow. Individual CFUs were harvested from mice receiving transplants of 100% MSH2–/– cells and subjected to PCR using a 33P-labeled primer specific for the informative locus D3mit 203. PCR products were electrophoresed on a 7% denaturing gel. The representative gel depicts characteristic microsatellite instability in 2 of 13 CFUs (lanes 3 [insertion] and 12 [deletion]) from secondary recipient mice. Similar evaluations of wild-type marrow from primary and secondary recipients failed to show evidence of microsatellite instability.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/5/10.1182_blood-2002-10-3035/6/h81734841004.jpeg?Expires=1735582967&Signature=faOY6QdmCS0VsYRh0UzrvrWx262oOVDCiqPU9IO1IWxckvqlt0hnypVxeB~mDrP1Xp0cvRDLg3KjBZ4F~GnSn7V9P7TIDJV1s7v3a06Ax3QQi7Yu5XNhOfn7avo5vF5HFEWlQgj~7n7YYMvqN2r~DjVlzZ6KJdwX7ZmioBVd97RQ0NXdHLbatjwSRLahGM9O4v6wBdXPbt2WZTkJQjPECUYPMfmFepIVsKEpr7xAs6O8Ah2tUlpYHLc8wN9S8J9HVsoJORh9PrLdc26iCaPBRMoJVprvsOxZSiRCvPUH7VLOL12QH9FDWVxFf4d2DXJU63W-fRJpVa5rRKGTicjQOw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Microsatellite instability in CFUs isolated from mice receiving MSH2–/– bone marrow. Individual CFUs were harvested from mice receiving transplants of 100% MSH2–/– cells and subjected to PCR using a 33P-labeled primer specific for the informative locus D3mit 203. PCR products were electrophoresed on a 7% denaturing gel. The representative gel depicts characteristic microsatellite instability in 2 of 13 CFUs (lanes 3 [insertion] and 12 [deletion]) from secondary recipient mice. Similar evaluations of wild-type marrow from primary and secondary recipients failed to show evidence of microsatellite instability.
