Frequency of BMLF1- or pp65-specific cells among CD8⁺ GMCs or CD8⁺ Co cells. PBMCs, Co cells, and GMCs were stained with CD8-PC5 mAb and BMLF1 or pp65 tetramers to assess the percentage of tetramer⁺ cells among CD8⁺ cells. The frequency of tetramer⁺ cells within CD8⁺ PBMCs was set as the 100% reference (dotted line). Data are expressed as the variation (mean ± SE) of the percentage of tetramer⁺ cells in CD8⁺ GMCs or CD8⁺ Co cells, as compared with the CD8⁺ PBMC reference. ▪ indicates results obtained with G418-selected GMCs and their respective Co cells (n = 3); and ▧, results from NGFR-selected GMCs and their respective Co cells (n = 3).