Spontaneous and activation-induced cell death of BMLF1- and pp65-specific CD8⁺ T cells. PBMCs from HLA-A2⁺ double seropositive donors were cultured in the absence (white symbols) or presence (black symbols) of CD3 mAb and 500 UI/mL IL-2. At the indicated time, cells were stained with annexin-V–FITC, CD8-PC5 mAb, and PE-labeled BMLF1 (squares) or pp65 (triangles) tetramers. Data are expressed as the percentage of annexin-V⁺ cells within the tetramer⁺ cells (mean ± SE, n = 5).