Figure 10.
Expansion of CD8+CD27+ and CD8+CD27– cells from day 0 to day 12. CD27+ cells were immunomagnetically purified from PBMCs, and the CD27+ (□) and CD27– (▪) fractions were activated by CD3 mAb and cultured in the presence of 500 UI/mL IL-2 until day 12. At the indicated time, cells were stained with CD8-PC5 and numerated to determine relative cell number (mean ± SE, n = 5).