Expression of chemokines, chemokine receptors, and IL-7 in the LckOM LN. Expression of 10 chemokines, 9 chemokine receptors, and IL-7 was assessed by semiquantitative RT-PCR done in triplicate in at least 3 independent experiments. The level of cDNA of interest relative to the level of 18S was estimated in the thymus and mesenteric LNs of B6 mice and in the mesenteric LNs of LckOM mice (12 weeks of age). (A) For 9 chemokine and 9 chemokine receptor genes, results are depicted in a histogram as the mean ± SD. Expression level in B6 LNs was arbitrarily defined as 1 and the relative expression in B6 thymus and LckOM LN is depicted as B6 thymus/B6 LN and LckOM LN/B6 LN ratios, respectively. Phosphoimagery of (B) CCL20 and (C) CCL25 expression. (D) Expression of IL-7. In panels A and D, ▪ indicates LckOM LN/B6 LN; □, B6 thymus/B6 LN; and ▦, LckOM spleen/B6 spleen.