American Society of Hematology

Figure 5.

$Figure 5. Expression of CCL20 transcripts in lymphoid and nonlymphoid organs. (A) CCL20 mRNA expression was estimated in various organs of B6 (□) and LckOM (▪) mice by semiquantitative RT-PCR. Results are shown as the ratio of CCL20 expression over internal control (18S) and represent the mean ± SDof3to9 mice per organ. si indicates small intestine; lgi, large intestine; stom, stomach; kid, kidney; and pp, Peyer patches. No significant difference was found between B6 versus LckOM organs (Mann-Whitney U test and Student t test). (B) CCL20 is produced by nonlymphoid stromal cells in the B6 thymus and LckOM LN. RNA was extracted from the B6 thymus and LckOM LN as follows: total organ, a cell suspension (cells) obtained after mechanical disruption of the thymus or LN through a cell strainer, and the remaining nonsuspendable fraction (stroma). Cell suspensions from collagenase-treated organs were sorted into T cells (Thy1.2+), B cells (CD19+), and DCs (CD11c+MHC II+). Serial 1:5 dilutions of each cDNA were used as template in semiquantitative RT-PCR. Representative results of 3 experiments are shown.$

Expression of CCL20 transcripts in lymphoid and nonlymphoid organs. (A) CCL20 mRNA expression was estimated in various organs of B6 (□) and LckOM (▪) mice by semiquantitative RT-PCR. Results are shown as the ratio of CCL20 expression over internal control (18S) and represent the mean ± SDof3to9 mice per organ. si indicates small intestine; lgi, large intestine; stom, stomach; kid, kidney; and pp, Peyer patches. No significant difference was found between B6 versus LckOM organs (Mann-Whitney U test and Student t test). (B) CCL20 is produced by nonlymphoid stromal cells in the B6 thymus and LckOM LN. RNA was extracted from the B6 thymus and LckOM LN as follows: total organ, a cell suspension (cells) obtained after mechanical disruption of the thymus or LN through a cell strainer, and the remaining nonsuspendable fraction (stroma). Cell suspensions from collagenase-treated organs were sorted into T cells (Thy1.2⁺), B cells (CD19⁺), and DCs (CD11c⁺MHC II⁺). Serial 1:5 dilutions of each cDNA were used as template in semiquantitative RT-PCR. Representative results of 3 experiments are shown.

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