Cyclin D1 mRNA levels in 46 MM patients who were investigated for 11q abnormalities and 10 healthy controls. The dashed line at ΔΔC_t = 19.5 indicates the cut-off level chosen to define cyclin D1 overexpression (ie, < 19.5). Patients with the t(11;14) had significantly lower ΔΔC_t values (reflecting higher cyclin D1 levels) than patients with trisomy 11 (+11) (P = .001); both of these subgroups significantly overexpressed cyclin D1 in comparison with patients without 11q abnormalities (P < .0001) and with healthy controls (P < .0001).