Increased oxidizability of HbLDL: effect of heme binding and LOOH reduction. (A) Auto-oxidation of nLDL and HbLDL. LDL samples were diluted to 0.25 mg/mL and conjugated diene formation was monitored at 234 nm at 2-minute intervals at 22°C using a Beckman DU-650 spectrophotometer (Beckman Coulter). (B) Copper-mediated oxidation of nLDL and HbLDL in the absence and presence of 10 μM KCN. (C) The effect of ebselen on copper-mediated oxidation of nLDL and HbLDL. nLDL and HbLDL were pretreated with ebselen in the presence of glutathione as described in “Materials and methods.” The conjugated dienes were measured at 234 nm.