Effect of ebselen treatment on HO-1 induction by different oxidized LDLs or hemin. LDLs (1 mg) were incubated with 50 μM ebselen in the presence of 3 mM glutathione, dialyzed, and LOOH levels measured. RAECs were incubated with indicated concentrations of nLDL, HbLDL, CuLDL, LDL^–, and hemin, with (□) or without (▪) ebselen pretreatment. After 24 hours cells were harvested and HO-1 protein was visualized by immunoblotting. Densities were calculated and data normalized based on β-actin levels. The figure shows one representative picture reflecting typical changes.