Most of CMV-specific CD8⁺ T cells are found among pE₂ and effector subsets. (A) Phenotypic analysis of purified CD8⁺ T cells from a healthy CMV carrier following staining with CMV multimers and the indicated mAbs. (B) Gene expression analysis on sorted RA⁺CCR7^–27⁺ (pE) and RA⁺CCR7^–27^– (E) CMV⁺ T-cell subsets by RT-PCR. The same set of primers as described in Figure 2B was used. Data from 7 independent 5-cell aliquots are shown. The phenotype as well as the pattern of gene expression of these CMV-specific T cells was stable over an interval of 8 months. The (–) indicates negative and (+) positive controls.