Analysis of O-glycans by HPAEC-PAD. Glycans released by alkaline-borohydride treatment from delipidated splenocyte membrane glycoproteins were analyzed by HPAEC-PAD. (A) β4GalT-I^+/– splenocytes. (B) β4GalT-I^–/– splenocytes. (C) Proposed structures of the O-glycans (A-B). The major glycan peaks are numbered in panels A and B, and the numbers in parentheses in panel C represent the peak numbers in panels A and B.