Analysis of desialylated O-glycans by HPAEC-PAD. Desialylated O-glycans from splenocyte membrane glycoproteins were analyzed by HPAEC-PAD. (A) β4GalT-I^+/– splenocytes. (B) β4GalT-I^–/– splenocytes. The major glycan peaks are numbered (A-B), and their proposed structures are given in the figure.