Peripheral blood hematology and the colony-forming activity of bone marrow cells. (A) Total white blood cell counts, red blood cell counts, hematocrit values, and hemoglobin concentrations of the peripheral blood from β4GalT-I+/– (□;n = 6) and β4GalT-I–/– mice (▦; n = 6) at 2 to 3 months of age were measured by an automatic cell counter. (B) Numbers of neutrophils, lymphocytes, and monocytes from β4GalT-I+/– (□; n = 6) and β4GalT-I–/– (▦; n = 6) mice were measured manually. (C) CFUs of nonadherent mononuclear cells (1 × 104) prepared from the bone marrow of β4GalT-I+/– (□, n = 5) and β4GalT-I–/– (▦; n = 5) mice were measured in the presence of 50 ng/mL G-CSF, 2 U/mL EPO, or 20 ng/mL IL-3. (D) Weights of spleens, PLNs (cervical LN + axillary LN), and mesenteric (M) LNs were compared between β4GalT-I+/– (□;n = 9) and β4GalT-I–/– (▦; n = 8) mice at 2 to 4.5 months of age. #P < .005; *P < .01; +P < .05. Data are presented as means ± SEM.
