Separation of patients based on their expression of the 35 prognosis-associated genes. (A) Hierarchical clustering of 54 patients and 4 NBM controls based on the expression of 35 prognosis-associated genes. Patients were divided into 2 major clusters indicated by red and blue bars (high-risk and low-risk groups, respectively). (B) Kaplan-Meier plots of EFS of patients divided into high-risk and low-risk groups based on the expression of the 35 prognosis-associated genes (GP and PP patients were excluded). EFS differed significantly between the 2 groups (P = .03 in log-rank test). (C) PCA of 54 patients and 4 NBM controls based on the expression of 35 prognosis-associated genes. The first and second components, which represent 31.8% and 12.5%, respectively, are shown. Red and blue closed circles indicate the PP and GP patients, respectively. Red and blue open circles indicate other patients who experienced relapse and those who did not experience relapse, respectively. Green closed circles indicate NBM controls. Patients who had relapses, including PP patients, those who did not have relapses, including GP patients, and NBM controls were clustered separately.