Localization of P210^BCR-ABL to F-actin is dependent on the presence of the coiled-coil domain and the C-terminal actin-binding domain. NIH 3T3 cells were transduced with pK1, wild-type P210^BCR-ABL, ΔActin BCR-ABL, Δ(1-63) BCR-ABL, or (1-63) BCR-ABL to determine the essential regions for colocalization of BCR-ABL with F-actin. Puromycin-resistant NIH 3T3 cells were stained for BCR-ABL expression (anti-abl, cy3, red), F-actin (fluorescein isothiocyanate (FITC)–phalloidin, green), and the nucleus was visualized by staining with DAPI (blue).