Figure 4.
Migration of peripheral and decidual NK cells to recombinant human CXCL9, CXCL10, and CXCL12-α. Migration assays were performed as described in “Materials and methods.” The amount and identity of the migrated NK cells was analyzed by flow cytometry. Dose-dependent preferential migration of peripheral (left column) and decidual (right column) NK cells to CXCL9 (A-B), CXCL10 (C-D), and CXCL12-α with or without T140 blocking of CXCR4 (E-F). The percentages of migrated cells are calculated from the total cell population after the subtraction of the spontaneous migration. R-squared value for all linear trend lines displayed was above 0.95; *indicates P < .001 by Student t test. One representative experiment is shown of 4 performed. Error bars indicate SD.