Enrichment of the CD16^– subset in the migrated peripheral NK cells to CXCL12-α. The presence of the various NK populations in the migrated NK cells presented in Figure 4 was analyzed by quadruple staining. The percentage of increase or decrease of the CD16^– or CD16⁺ subsets was calculated with regard to the starting point when no chemokine was present (100%). Migration was performed to CXCL9 (A), CXCL10 (B), and CXCL12-α (C). (D-G) Contour histogram of the CD16 phenotype of the migrated NK cells to CXCL9 (E), CXCL10 (F), and CXCL12-α (G). Control (D) represents the spontaneous migration when no chemokine was added. The percentages of CD16^– and CD16⁺ subsets in the NK cells that spontaneously migrated when no chemokine was present were similar to those observed in the peripheral blood. Figure shows 1 representative experiment of 3 performed.