Expression of various chemokine receptors on cytokine-activated peripheral CD16^– NK cells. PBLs were obtained from healthy donors and were either left untreated or incubated with various cytokines at a concentration of 100 ng/mL and then were quadruple stained for the expression of chemokine receptors on CD16^– NK cells. (A) No cytokine treatment, (B) IL-8, (C) IL-10, (D) IL-12, (E) IL-15, (F) TGFβ1, and (G) IL-8 + IL-10 + IL-12 + IL-15 + TGFβ1. The indicated values represent the percentage of cells positive for a certain chemokine receptor of the total population. One representative experiment is shown of 3 performed.