Figure 2.
Ectopic expression of Hsp70 augments DNA fragmentation induced by staurosporine and anti-CD3/CD28 Abs in TAg-Jurkat cells. Cells (5 × 105) of GFP transfectant (GFP-3) or Hsp70 transfectant (Hsp70-46) were treated with various concentrations of staurosporine (STS) (A) or anti-CD3/CD28 Abs (B) and DNA fragmentation was analyzed by electrophoresis on a 1.5% agarose gel. DNA was visualized with ethidium bromide (left panel). The intensity of the fluorescence of unfragmented DNA (a) and that of fragmented DNA (b) was measured by densitograph software (Atto, Tokyo, Japan), and the ratio of fragmented DNA and total DNA, b/(a+b), is shown on the right panel. Results are shown as mean ± SD of 3 independent experiments. *P < .05; **P < .01.
