Stabilization of CAD activity by Hsp70. Caspase-3–activated CAD was incubated without (–) or with either purified Hsp70 preparation (1 μL) or IgG immunoprecipitates (1 μL, control) in reaction buffer at 37°C for various times, and CAD activity was analyzed as in Figure 4. Initial CAD activity at 0 minute was indicated as 100%. Relative CAD activity is shown. Results are demonstrated as the mean ± SE of 2 independent experiments.