DNA-fragmentation activity of the Hsp70/CAD complex in the cells. Stimulated with staurosporine were the 293T cells that had been transfected with either Flag-Hsp70 or its mutant expression vectors (Hsp70ΔABD, Hsp70ΔPBD) together with HA-CAD and ICAD expression vectors. Hsp70 or its mutant proteins were immunoprecipitated as in Figure 5 B-C, and CAD activity was analyzed as in Figure 5D in the absence or presence of ICAD (lanes 1-6). To confirm the inhibitory activity of ICAD, caspase-3–activated CAD was incubated with or without ICAD and then DNA-fragmentation activity was analyzed (lanes 7-8).