Figure 5.
Figure 5. Thiol reagents differentially inhibit platelet adhesion to GFOGERGPP, collagen, and CRP. (A) Platelet adhesion to the 3 substrates was measured, as described, in the presence of increasing levels of DTNB. Data (mean ± SD) are from 3 different experiments performed in triplicate using adhesion method B. (B) Platelet adhesion to GFOGDR-GPP and to GPP10 was compared with adhesion to GFOGER using method B in a single experiment repeated in triplicate (mean ± SD) confirming previously published data.38,42 (C) Surfaces were coated with GFOGER, as for panel A, then pretreated for 30 minutes with 10 mM DTNB (▪) or the vehicle for DTNB, phosphate buffer (▦). After washing the surfaces with phosphate buffer, platelets were added in the presence of the indicated level of DTNB and adhesion was measured as for panel A. (D) Platelet adhesion to the 3 substrates was measured, as described, in the presence of increasing levels of pCMPS. Data are from 3 separate experiments, each performed in triplicate (mean ± SD), using adhesion method A. Where error bars are absent, they were too small to show.

Thiol reagents differentially inhibit platelet adhesion to GFOGERGPP, collagen, and CRP. (A) Platelet adhesion to the 3 substrates was measured, as described, in the presence of increasing levels of DTNB. Data (mean ± SD) are from 3 different experiments performed in triplicate using adhesion method B. (B) Platelet adhesion to GFOGDR-GPP and to GPP10 was compared with adhesion to GFOGER using method B in a single experiment repeated in triplicate (mean ± SD) confirming previously published data.38,42  (C) Surfaces were coated with GFOGER, as for panel A, then pretreated for 30 minutes with 10 mM DTNB (▪) or the vehicle for DTNB, phosphate buffer (▦). After washing the surfaces with phosphate buffer, platelets were added in the presence of the indicated level of DTNB and adhesion was measured as for panel A. (D) Platelet adhesion to the 3 substrates was measured, as described, in the presence of increasing levels of pCMPS. Data are from 3 separate experiments, each performed in triplicate (mean ± SD), using adhesion method A. Where error bars are absent, they were too small to show.

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