Figure 10.
![Figure 10. TAT-NBD inhibited basal and LPS-induced IKK activity in a dose-dependent manner. The effect of TAT-NBD on constitutive and LPS-induced IKK activity was studied with in vitro kinase assay. Cells (6 × 106) pretreated with either buffer control, TAT-NBD (NBD), or the mutant control (MUT) were stimulated with 1 μg/mL LPS or left untreated. Whole cell lysates were immunoprecipitated with a monoclonal IKKα antibody. Precipitates were incubated with 1 μg GST-IκBα and 3 μCi (0.111 MBq) [γ-32]ATP. Samples were boiled and subjected to 12% SDS-PAGE and autoradiography. Equal loading was confirmed by IKKα Western blot.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/6/10.1182_blood-2002-09-2960/6/h81834919010.jpeg?Expires=1736062772&Signature=Hw6sdvhDJCHZk6Ktoyku8lcwGE8bxQlbZ50ZRsAVRp3LUVQ3OgYKGuK9EwGYet53MZBOqiv55tzZThqMm2e7uOzUJVPgenn75Capobu4lnqqJrXVoyWe1wU7xhP7m51lgCVPNA9RS4D7P7-uFLbGp94E9rjbSiWQerQMEfDZMMR5tt4EnWHxkIX-f5ZWPdaKSO4lKljSzZk8cHqci-qxczOMDtcMykCPLgPG-0MS99nWJ-cMLht2iZMACNwB7F2OL97GtslLXxd5ojQYpxLHuDE3jGpY5XjmDoXoQa-9-wZ4mrcSYAEA5c2UJfxBJ3YEZLaU1s6IAuz1IOPwWiEGgg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TAT-NBD inhibited basal and LPS-induced IKK activity in a dose-dependent manner. The effect of TAT-NBD on constitutive and LPS-induced IKK activity was studied with in vitro kinase assay. Cells (6 × 106) pretreated with either buffer control, TAT-NBD (NBD), or the mutant control (MUT) were stimulated with 1 μg/mL LPS or left untreated. Whole cell lysates were immunoprecipitated with a monoclonal IKKα antibody. Precipitates were incubated with 1 μg GST-IκBα and 3 μCi (0.111 MBq) [γ-32]ATP. Samples were boiled and subjected to 12% SDS-PAGE and autoradiography. Equal loading was confirmed by IKKα Western blot.
