Surface expression of HLA-specific NK receptors in polyclonal NK cell lines from LDGL patients. Polyclonal NK cell lines (NKLGL) derived from 7 LDGL patients and from 2 controls were stained with the indicated mAb specific for different NKRs. In particular, EB6b mAb reacts with KIR2DL1 and S1, GL183 mAb reacts with KIR2DL2/3 and S1, FES172 mAb reacts with KIR2DS4, Z27 mAb reacts with KIR3DL1, Z199 mAb reacts with NKG2A, and F278 mAb reacts with LIR1/ILT2. Open profiles indicate the isotype controls, and gray profiles refer to the specific fluorescence with the above mAbs, whereas black profiles bring into evidence the homogeneous reactivity of given anti-KIR mAb with the whole NK cell population.