Effect of CD2 blockade on DC-induced CD4⁺CD45RA+ T-cell activation and cytokine production. The pDCs were cultured at 4°C in the presence of anti-T11₁ or an IR mAb. After 30 minutes, preparations were washed 3 times and resuspended in culture medium. These pulsed and unpulsed pDCs (10⁴) were separately cocultured with CD4⁺CD45RA+ T cells (105), and their ability to induce allogeneic and autologous T-cell (Tc) proliferation was measured (A). Results are expressed as the percent proliferation and are typical of data obtained from 3 similar experiments. Parallel samples were cultured, as stated in “Materials and methods,” harvested, and stained with anti–IL-2 and anti–IFN-γ (B). In panel B, ▪ indicates control; □, Gag; and ▧, TT. P values were calculated from intracellular cytokine data from 3 independent experiments.