Summary of MPD marker analysis. (A) Cohort of 44 MPD patients. Open squares signify normal findings, black squares indicate abnormal values. Expression of c-MPL protein from platelets was detected by Western blot analysis and compared with CD61 to normalize for platelet protein loading. Unique patient numbers were placed above the corresponding lanes. L indicates decreased expression of c-MPL protein. PRV-1 and NFIB mRNA levels were determined by real-time PCR. Numbers indicate the ΔC_T values (see “Study design”). Note that high numeric ΔC_T values indicate low or normal abundance of mRNA (open squares), whereas low or negative ΔC_T values signify elevated levels of expression (black squares). Presence (+) or absence (–) of EEC or 9pLOH is indicated. nd indicates not determined; P, polycythemia vera; E, essential thrombocythemia; and M, chronic idiopathic myelofibrosis. (B) Healthy controls. (C) Hereditary thrombocythemia. Individuals within the pedigree are placed above the corresponding lanes and are numbered as by Wiestner et al.¹³  Black symbols indicate affected individuals. Serum TPO concentration in pg/mL (numbers in italic) and platelet count × 10⁹/L (numbers in regular style) are shown below each individual.