Figure 2.
Figure 2. FcR-γ levels in the absence of GP VI. Mouse platelet lysates were separated on a 4% to 20% SDS-PAGE gel under reducing conditions and immunoblotted with anti–FcR-γ chain antiserum. The same membrane was subsequently reacted with an anti–14-3-3ζ polyclonal antibody to document the approximate protein load for each lane. Both antibodies were detected by HRP goat antirabbit IgG and an enhanced chemiluminescence detection system. Also shown for comparison is the platelet lysate from FcR-γnull platelet lysates. The 14-3-3ζ is seen at 32 kDa and FcR-γ is seen at 7 kDa.

FcR-γ levels in the absence of GP VI. Mouse platelet lysates were separated on a 4% to 20% SDS-PAGE gel under reducing conditions and immunoblotted with anti–FcR-γ chain antiserum. The same membrane was subsequently reacted with an anti–14-3-3ζ polyclonal antibody to document the approximate protein load for each lane. Both antibodies were detected by HRP goat antirabbit IgG and an enhanced chemiluminescence detection system. Also shown for comparison is the platelet lysate from FcR-γnull platelet lysates. The 14-3-3ζ is seen at 32 kDa and FcR-γ is seen at 7 kDa.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal