Liver sections stained with antistomatin antibody. Healthy control and OHSt patient. Detail of liver parenchyma showing the trabeculae of hepatocytes and sinusoids. Upper panels (A-B): normal liver; lower panels (C-E): patient A-I-2. (A) Stomatin immunoreactivity in normal liver is associated to the plasma membrane of the hepatocytes. Note the positive staining of red and white blood cells in the sinusoids. (B) Normal liver, negative control, and omitting antistomatin antibody. (C) Patient A-I-2: antistomatin antibody, nuclei counterstained with hematoxylin. As in the control, stomatin immunoreactivity is found associated to the plasma membrane of hepatocytes. Sinusoids are nearly free from red cells due to the fatal hemolytic crisis but show some immunopositive white blood cells. (D) Patient A-I-2: negative control, omitting antistomatin antibody, showing discrete granular brownish deposits of hemosiderin in the cytoplasm. Nuclei were counterstained with hematoxylin. (E) Stomatin immunostaining combined with Prussian blue reaction. Note the blue ferrocyanide-positive deposits in the cytoplasm and the brownish stomatin immunoreaction product that is accentuated at the plasma membrane (arrow). Bar represents 10 μm.