Induction of T-cell proliferation by immature and mature MoDCs and CD1c⁺ PBDCs. Immature MoDCs (GM-CSF plus IL-4) or freshly sorted CD1c⁺ PBDCs were stimulated for 2 days with the indicated stimuli, washed, and used as stimulators (1 × 10⁴) of alloreactive T cells (1 × 10⁵) in the MLR. On day 5 of the MLR, supernatants were harvested, and fresh medium containing 1 μCi (0.037 MBq) [³H]thymidine was added to each well for 8 hours. Proliferation of T cells stimulated with graded numbers of MoDCs (A) or CD1c⁺ PBDCs (B) is shown. Data represent the means ± SEMs of triplicate wells. This figure is representative of experiments from 5 separate donors.