Figure 1.
Figure 1. Schematic representation of the first BCL-6 intron and derivative chromosomes after t(3;14) translocation. Breakpoints on der(3q27) are indicated (vertical arrows) for each case and located according to the MMC germ line sequence (GenBank accession no. AF191831). MTC or MMC forward (F) and reverse (R) primers, previously designed,9,16 were used to amplify the junction site and the translocated allele in combination with Switch μ or Switch γ primers (Sμ 01F 5′-ggcaatgagatggctttagctg, Sμ 02F 5′-tgggctgagcaggttgtac, Sμ 01R 5′-tgccatgtccttccagttcatcc, SγF 5′-gggcagaatggtcataat, SγR 5′-atgttctcccctgttccctgag). To exclusively amplify the untranslocated allele, MTC or MMC primers spanning the breakpoints were used under PCR conditions identical to those used for amplification of the BCL6-IgH junctions.

Schematic representation of the first BCL-6 intron and derivative chromosomes after t(3;14) translocation. Breakpoints on der(3q27) are indicated (vertical arrows) for each case and located according to the MMC germ line sequence (GenBank accession no. AF191831). MTC or MMC forward (F) and reverse (R) primers, previously designed,9,16  were used to amplify the junction site and the translocated allele in combination with Switch μ or Switch γ primers (Sμ 01F 5′-ggcaatgagatggctttagctg, Sμ 02F 5′-tgggctgagcaggttgtac, Sμ 01R 5′-tgccatgtccttccagttcatcc, SγF 5′-gggcagaatggtcataat, SγR 5′-atgttctcccctgttccctgag). To exclusively amplify the untranslocated allele, MTC or MMC primers spanning the breakpoints were used under PCR conditions identical to those used for amplification of the BCL6-IgH junctions.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal