Morphology and IgE receptor expression. (A) Cytospin preparations of 4-week-old HF cultures were stained with the indicated dyes. Scale bars represent 10 μm. (B) The same samples were double-stained with anti–c-kit mAb and anti-IgE mAb after 30 minutes of incubation with or without IgE. (C) Data shown are the staining profiles with anti-IgE mAb within the c-kit⁺ populations of the HF cultures (left) and for Pam 212 keratinocytes (right) after preincubation with (closed histograms) or without (open histograms) IgE. Data shown are representative of 3 independent experiments.