Surface phenotype of HF-derived leukocytes established in the presence of SCF alone. (A) Vibrissal follicles (intermediate fragments) were cultured for 8 weeks in the presence of 10 ng/mL SCF alone. Cells harvested from these cultures were stained for Lin, c-kit, and Sca-1. Data shown are the expression profiles for Lin and Sca-1 in nongated populations (left) and for Sca-1 and c-kit in the Lin^– populations (right). (B) The same preparations were stained with anti–c-kit mAb and anti-IgE mAb after 30 minutes of preincubation in the presence or absence of IgE. Three HF-derived leukocyte cultures established independently in the presence of SCF alone all showed virtually indistinguishable surface phenotypes.