Figure 1.
![Figure 1. Fluorescent genescan detection of FLT3-ITD. (A) Sensitivity of detection from cDNA (left column) versus DNA (right column). PCR products from a representative case (unique patient number [UPN] 529), after dilution into PBL DNA/RNA. The size of the ITD is given relative to the wild-type allele and the fluorescent scale indicated. (B) Subclone detection: minor subclones were more frequently detected from cDNA, as demonstrated for 2 representative patients. (C) Differences in relative intensity of the wild-type compared with FLT3-ITD alleles for cDNA versus DNA in a representative AML.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/6/10.1182_blood-2003-01-0162/6/h81834923001.jpeg?Expires=1735487509&Signature=LA0ZxLmxUTy2k8BBscrnEZfl4VqfzTkB0uEmZaRjZF7ykoUZUVRJCn~Z7w0~E73ZJnfpzi5ARYaQ7LN053dBOUgfUEkXWtWxGyatOsyozvcc6i0krpE-PVNI3lHWedsAdIAv1ErosTC7fPayQ~KNQjjt2aJrvdLV6WLlosPK49byfksxmCkCGb~DvY8TvDhXfZ9HxtSEsAhlxoQd0ZEl5A3IIC~bvkpSGbaJmTgIP-Zs5DgSgLjDfwlRgkdUGORYVK1pFOxxjJfTmOmhguXuluJ7CiFiyJf0Le0ZTh9oWMl99zU9VC1uS7scNmo5qn-u1NozZvo48b3Cvq-~5h-n8A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fluorescent genescan detection of FLT3-ITD. (A) Sensitivity of detection from cDNA (left column) versus DNA (right column). PCR products from a representative case (unique patient number [UPN] 529), after dilution into PBL DNA/RNA. The size of the ITD is given relative to the wild-type allele and the fluorescent scale indicated. (B) Subclone detection: minor subclones were more frequently detected from cDNA, as demonstrated for 2 representative patients. (C) Differences in relative intensity of the wild-type compared with FLT3-ITD alleles for cDNA versus DNA in a representative AML.
