LTC-DCs resemble CD8α^– myeloid-like DCs. Cells were labeled with antibodies specific for DC markers using an indirect 2-color staining technique. Cells were separated into small and large LTC-DC subsets using postacquisitional gating based on forward scatter and side scatter. Quadrants distinguishing positively and negatively staining cells were placed to represent background staining with only labeled conjugate. Data are representative of many separate experiments. The quadrants for CD8α are set at a lower intensity because acquisition was performed using lower FL1 and FL2 voltages than were used for other markers.