Effect of FVIIa on cell viability. Monolayers of BHK(+TF) cells cultured to 80% confluency were incubated for 24 hours in serum-free medium (SFM) alone or SFM supplemented with FCS (10% vol/vol), FVIIa (10 nM), or FFR-FVIIa (10 nM). At the end of 24 hours, the overlying conditioned medium was removed and assayed for LDH activity. LDH activity obtained with SFM was set to 100%. Data are presented as mean ± SD (n = 3). Statistically significant differences from the value obtained in SFM are indicated by an asterisk (P < .001). NS indicates no statistically significant difference.