Antiapoptotic effect of FVIIa. (A) Monolayers of BHK(+TF) cells were exposed to SFM or the SFM supplemented with varying concentrations of FVIIa or serum (10% vol/vol) for 24 hours. At the end of the incubation period, fragmented DNA was labeled with TUNEL and detected by flow cytometry. (B) Wild-type BHK cells were exposed for 48 hours to SFM alone or to the medium supplemented with serum (10% vol/vol) or FVIIa (100 nM). At the end of the incubation period, cells were processed for TUNEL assay as described for panel A. Figures show a representative experiment (n = 3).