FVIIa induces phosphorylation of p44/42 MAPK and Akt in CHO(+TF) cells. Monolayers of quiescent CHO(+TF) cells were stimulated with SFM, FVIIa (50 nM), or serum (10% vol/vol) for the indicated times. Cell lysates were analyzed by Western blot analysis using phospho-specific antibodies against p44/42 MAPK and Akt. One representative experiment of 3 independent experiments is shown.