PI3K/Akt pathway is responsible for the antiapoptotic properties of FVIIa. Monolayers of BHK(+TF) cells were incubated in SFM alone or the SFM supplemented with FVIIa (50 nM) in the presence and absence of inhibitors. Inhibitors were added to the cells 30 minutes prior to the addition of FVIIa. After 4 hours, the cells were lysed and the cell lysates (10 μg) were subjected to Western blot analysis using anti–caspase 3 antibody. Panel A shows a representative Western blot analysis. Panel B represents quantification of band intensity obtained from digital pictures. Caspase 3 activation obtained with serum-free medium was set to 100% apoptosis. Concentration of inhibitors was LY294002 (5 μM), U0126 (5 μM), and AG1478 (10 μM). Data are presented as mean ± SD (n = 4). Statistically significant differences from FVIIa value are indicated. *P < .05; **P < .001; and NS indicates not significant.