Specific action of PI3K and MEK1/2 pathway inhibitors on activation of Akt and p44/42 MAPK. Monolayers of BHK(+TF) cells were made quiescent by serum deprivation for 2 hours. Then the cells were treated with FVIIa in the presence and absence of inhibitors as described in Figure 7. After 10 minutes, the experiment was terminated by lysing the cells. The cell lysates (10 μg) were subjected to Western blot analysis using phospho-specific antibodies against p44/42 MAPK and Akt. The figure shows one representative Western blot of 4 independent experiments.