Migration and adhesion of cord blood CD34⁺ cells to supernatants from untreated and TGF-β 1–treated MS-5 cells. CD34⁺ cells isolated from human cord blood samples were preincubated with T134 and subjected to chemotaxis (A, left) or transendothelial migration across HUVECs (A, right) to supernatants from MS-5 cells treated with or without TGF-β 1 (+ TGF-β 1 and –TGF-β 1, respectively). Data represent the mean ± SD of 6 (left) and 5 (right) experiments. Reduction in cell migration was significant (**P < .005; *P < .05), according to Student 2-tailed t test. (B) BCECF-AM–labeled CD34⁺ cells were preincubated with T134 and subsequently exposed for 1 minute at 37°C to supernatants from MS-5 cells incubated with or without TGF-β 1. Cells were added to wells containing sVCAM-1 and were subjected to adhesion for 4 minutes after a short spin. Nonbound cells were washed, and the extent of adhesion was measured in a fluorescence analyzer. Data represent the means ± SDs of triplicate samples from a representative result of 3 experiments. Reduction in triggering of cell adhesion was significant (**P < .005), according to Student 2-tailed t test.