Figure 1.
Secretory machinery in mouse platelets. Platelets were collected from platelet-rich plasma (PRP) and washed twice in Tyrode buffer with 0.18% sodium citrate. During the final wash, platelets were counted and resuspended to a concentration of 1.0 × 107/μL in 2 × SDS-PAGE sample buffer containing 1 mM EDTA, 2 × complete EDTA-free protease inhibitor, 300 μM calpeptin, 100 μM caspase 3 inhibitor, and 100 μM cathepsin inhibitor. Platelet equivalents (5.0 × 107 per lane) were loaded and analyzed by Western blotting with antibodies to the indicated proteins. The blots labeled VAMP 3 and VAMP 3* were exposed for 5 minutes and 5 hours, respectively.
