Synaptobrevin/VAMP-2 is present in human platelets. Human platelets were prepared from freshly drawn blood or from banked units and solubilized with 1 × SDS-PAGE buffer containing 2 × Roche Complete EDTA-free protease inhibitor, 2 mM EDTA, 2 mM EGTA, 300 μM calpeptin, 100 μM caspase 3 inhibitor, and 100 μM cathepsin inhibitor. Samples were then heated to 42°C for 20 minutes, and the indicated amounts of total proteins were analyzed by electrophoresis and Western blotting. As a blotting control, 1 μg mouse cerebrum was used.