American Society of Hematology

Figure 3.

$Figure 3. Loss of MMP. (A) U937 cells were exposed to 400 nM 17-AAG with or without 75 nM UCN-01 for 30 hours, after which the loss of MMP (Δψm) was determined by monitoring uptake of DiOC6 by flow cytometry as described in “Materials and methods.” Values represent the means ± SD for 3 separate experiments performed in triplicate. (B-C) U937 cells were exposed to varying concentration of UCN-01 and 17-AAG at a fixed ratio (1:5) for 30 hours, after which loss of MMP (Δψm) and the extent of apoptosis determined by monitoring DiOC6 uptake and annexin V/PI staining, respectively. Combination index values for each fraction affected were determined using commercially available software (Calcusyn; Biosoft). Combination index values less than 1.0 correspond to synergistic interactions.$

Loss of MMP. (A) U937 cells were exposed to 400 nM 17-AAG with or without 75 nM UCN-01 for 30 hours, after which the loss of MMP (Δψ_m) was determined by monitoring uptake of DiOC₆ by flow cytometry as described in “Materials and methods.” Values represent the means ± SD for 3 separate experiments performed in triplicate. (B-C) U937 cells were exposed to varying concentration of UCN-01 and 17-AAG at a fixed ratio (1:5) for 30 hours, after which loss of MMP (Δψ_m) and the extent of apoptosis determined by monitoring DiOC₆ uptake and annexin V/PI staining, respectively. Combination index values for each fraction affected were determined using commercially available software (Calcusyn; Biosoft). Combination index values less than 1.0 correspond to synergistic interactions.

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