Shp-2 is necessary for hemangioblast (BL CFC) formation. (A) Day 3, 4, or 5 EBs were dissociated and plated into hemangioblast cultures. At least 2 independent experiments were conducted for each comparison and cultures were plated in duplicate or triplicate. Day 3: *P < .0001 comparing WT to Shp-2^Δ; **P < .0001 comparing Shp-2^R1 to Shp-2^Δ. Day 4: *P < .006 comparing WT to Shp-2^Δ and **P = .01 comparing Shp-2^R1 and Shp-2^Δ. (B) Representative secondary EB. (C) Representative hemangioblast (BL-CFC) colony. Error bars represent SEM.