LIF-stimulated phospho-Erk is lower and phospho-Stat3 is greater in Shp-2^Δ cells. (A) WT, Shp-2^Δ, and Shp-2^R1 ES cells were either unstimulated or stimulated with LIF (1000 U/mL) for 5 or 20 minutes followed by immunoblot analysis for p-Erk and Erk. The blot is a representation of 4 independent experiments. (B) Compilation of normalized p-Erk to Erk levels from 4 independent experiments following LIF stimulation in WT, Shp-2^Δ, and Shp-2^R1 cells; *P = .04 comparing Shp-2^Δ to WT, and **P = .03 comparing Shp-2^R1 to Shp-2^Δ. (C) WT, Shp-2^Δ, and Shp-2^R1 ES cells were either unstimulated or stimulated with LIF (1000 U/mL) for 5 or 10 minutes followed by immunoblot analysis for p-Stat3 and Stat3. The blot is a representation of 4 independent experiments. (D) Compilation of normalized p-Stat3 to Stat3 from 4 independent experiments following LIF stimulation in WT, Shp-2^Δ, and Shp-2^R1 cells; *P = .05 comparing Shp-2^Δ to WT, and **P = .05 comparing Shp-2^R1 to Shp-2^Δ.