Figure 4.
Figure 4. The integrin αvβ3/JAM-1 complex is disrupted on stimulation of HUVECs with bFGF. (A) Immunofluorescence images of serum-starved, untransfected HUVECs in the absence or presence of bFGF stained for JAM-1 and integrin β3. Scale bar represents 10 μm. (B) Coimmunoprecipitation of αvβ3 with JAM-1 from HUVECs treated with bFGF or RGDS peptide where indicated. HB67 (cIgG) was used as a control. IgG heavy chain—IgG(H)—bands indicate equal loading.

The integrin αvβ3/JAM-1 complex is disrupted on stimulation of HUVECs with bFGF. (A) Immunofluorescence images of serum-starved, untransfected HUVECs in the absence or presence of bFGF stained for JAM-1 and integrin β3. Scale bar represents 10 μm. (B) Coimmunoprecipitation of αvβ3 with JAM-1 from HUVECs treated with bFGF or RGDS peptide where indicated. HB67 (cIgG) was used as a control. IgG heavy chain—IgG(H)—bands indicate equal loading.

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