Increased trafficking of nonengrafting Sca-1⁺ lin^- CD62L⁺ or Sca-1⁺ lin^- CD49e^- cells to spleen 20 hours after transplantation. In one representative experiment shown in panels A and B, irradiated mice (950 cGy) underwent transplantation with 8 × 10⁴ CFSE-labeled Sca-1⁺ lin^– CD49e^– (non-ENG) or Sca-1⁺ lin^– CD49e⁺ (ENG) cells, were killed 20 hours later, and CFSE-positive donor cells were detected flow cytometrically as described in “Materials and methods.” Dot plots illustrate increased frequency of non-ENG cells (A) in spleen compared with that of ENG cell transplants (B). (C-D) The distribution of total recovered graft cells in vivo when total recoveries in individual experiments were normalized to 100%. n = 10 in panel C and n = 11 in panel D. *P < .05 when compared with similar tissues in ENG cell transplants.